EYA 3和TSH β在季节性发情和常年发情绵羊中的表达模式

doi:10.11843/j.issn.0366-6964.2018.02.005

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (2): 263-269.doi: 10.11843/j.issn.0366-6964.2018.02.005

EYA 3和TSH β在季节性发情和常年发情绵羊中的表达模式

夏青¹, 张效生², 刘秋月¹, 王翔宇¹, 贺小云¹, 郭晓飞¹, 胡文萍¹, 张金龙², 储明星^1*, 狄冉^1*

1. 中国农业科学院北京畜牧兽医研究所, 农业部动物遗传育种与繁殖重点实验室, 北京 100193;
2. 天津市畜牧兽医研究所, 天津 300381

收稿日期:2017-06-12 出版日期:2018-02-23 发布日期:2018-02-23
通讯作者: 储明星,博士,研究员,博士生导师,E-mail:mxchu@263.net;狄冉,博士,副研究员,硕士生导师,E-mail:dirangirl@163.com
作者简介:夏青(1990-),女,河北石家庄人,硕士生,主要从事动物分子育种研究,E-mail:1833457068@qq.com;张效生(1978-),男,宁夏石嘴山人,高级畜牧师,E-mail:zhangxs0221@126.com,Tel:010-62816001。
基金资助:
国家自然科学基金项目（31572371）；国家转基因科技重大专项（2016ZX08009-003-006；2016ZX08010-005-003）；国家肉羊产业技术体系专项（CARS-38）；中央级公益性科研院所基本科研业务费专项（Y2017JC24；2017ywf-zd-13；2013ywf-zd-1；2015ywf-zd-2；2015ywf-zd-8）；中国农业科学院科技创新工程（ASTIP-IAS13；CAAS-XTCX2016010-01-03；CAAS-XTCX2016010-03-03；CAAS-XTCX2016011-02-02）；宁夏农林科学院科技创新先导资金项目（DWJLC-2016001）；内蒙古自治区科技重大专项；农业科研杰出人才及其创新团队项目；国家万人计划科技创新领军人才项目；天津市科技计划项目（16ZXZYNC00050）

Expression Patterns of EYA 3 and TSH β in Seasonal Estrous and Year-round Estrous Sheep

XIA Qing¹, ZHANG Xiao-sheng², LIU Qiu-yue¹, WANG Xiang-yu¹, HE Xiao-yun¹, GUO Xiao-fei¹, HU Wen-ping¹, ZHANG Jin-long², CHU Ming-xing^1*, DI Ran^1*

1. Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Tianjin Institute of Animal Science, Tianjin 300381, China

Received:2017-06-12 Online:2018-02-23 Published:2018-02-23

摘要/Abstract

摘要：

本研究旨在揭示EYA 3和TSH β两个基因在绵羊季节性发情调控和繁殖时期转换中的作用。采用q-PCR技术分析眼缺失基因3（Eyes absent 3，EYA 3）与促甲状腺激素β亚基基因（Thyrotrophin β subunit，TSH β）在不同光照条件下（长光照和短光照）季节性发情的苏尼特羊（Sunite sheep，SNT）和不同繁殖时期（黄体期和卵泡期）常年发情的小尾寒羊（Small Tail Han sheep，STH）各组织中的表达模式。结果表明，EYA 3基因在两个品种绵羊各组织中广泛表达，TSH β基因仅在绵羊垂体中高表达；在绝大多数组织中，两基因在苏尼特羊中表达量为长光照（Long photoperiod，LP）高于短光照（Short photoperiod，SP），而在小尾寒羊中是黄体期高于卵泡期；SP转至LP时，苏尼特羊垂体中EYA 3与TSH β的表达量均升高，在长光照第3天（Long photoperiod 3，LP 3）EYA 3表达量达到最高，在LP 21时，TSH β表达量达到最高，EYA 3表达量提前于TSH β开始降低。本研究揭示了EYA 3与TSH β在常年发情和季节性发情绵羊中的表达谱特征，不同光照条件与不同繁殖时期绵羊松果体与垂体中上述基因的表达模式，暗示EYA 3可能也在松果体部位发挥作用，并对绵羊季节性发情进行调控，EYA 3与TSH β两个基因也可能参与发情时期的转换；SP转变为LP过程中，EYA 3早于TSH β发挥调控作用。

Abstract:

The aim of this study was to reveal EYA 3 and TSH β regulatory roles in seasonal estrus and reproduction stages transition.we investigated the expression patterns of eyes absent 3 (EYA 3) and thyrotropin beta subunit (TSH β) genes in different tissues of seasonal estrous sheep(Sunite sheep, SNT)at different photoperiods (long photoperiod (LP) and short photoperiod(SP)) and year-round estrous sheep (Small Tail Han sheep, STH) at different reproduction stages (luteal phase and follicular phase) through q-PCR technology. The results showed that EYA 3 was widely expressed in different tissues of 2 breeds, while TSH β was expressed highly in pituitary. In most tissues, the expression levels of those 2 genes under long photoperiod (LP) were higher than those under short photoperiod (SP) in SNT, and the expression levels were higher at luteal phase than those at follicular phase in STH. When SP turned into LP in SNT, the expression levels of those 2 genes rose in pituitary, and EYA 3 showed a transient peak at LP 3, and the peak of TSH β was at LP 21. The expression of EYA 3 declined prior to TSH β. The results elucidated the expression characteristics of EYA 3 and TSH β in year-round estrous and seasonal estrous sheep, the expression characteristics of those 2 genes at different photoperiods and different reproduction stages in pineal and pituitary of sheep, which suggested that EYA 3 played an important role in pineal and might regulate seasonal estrous of sheep. In addition, EYA 3 and TSH β might be involved in the transition of estrous stages. When SP turned to LP, EYA 3 played a regulatory role earlier than TSH β.

中图分类号:

S826.2

夏青, 张效生, 刘秋月, 王翔宇, 贺小云, 郭晓飞, 胡文萍, 张金龙, 储明星, 狄冉. EYA 3和TSH β在季节性发情和常年发情绵羊中的表达模式[J]. 畜牧兽医学报, 2018, 49(2): 263-269.

XIA Qing, ZHANG Xiao-sheng, LIU Qiu-yue, WANG Xiang-yu, HE Xiao-yun, GUO Xiao-fei, HU Wen-ping, ZHANG Jin-long, CHU Ming-xing, DI Ran. Expression Patterns of EYA 3 and TSH β in Seasonal Estrous and Year-round Estrous Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 263-269.

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EYA 3和TSH β在季节性发情和常年发情绵羊中的表达模式

Expression Patterns of EYA 3 and TSH β in Seasonal Estrous and Year-round Estrous Sheep

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